Cell Ranger3.0, printed on 03/25/2023
The cellranger vdj pipeline will generate
.vloupe files for Single Cell
V(D)J experiments. A
.vloupe file will be found in the
outs folder of
a completed cellranger vdj run.
.vloupe file contains information about clonotypes and consensus alignments,
but does not contain the contig BAM file necessary to validate read support with the integrated IGV.js viewer.
all_contig.bam file is not present in the same folder as the
.vloupe file, you will be prompted
to enter either its location on a filesystem, or a web address where the file can be found. If you are
interested in validating read support, it may be worth it to keep the
in the same folder, though the
all_contig.bam file can grow very large.
You will still need to consult the Cell Ranger run summary file to view quality control data and other metadata about a particular run; this is not yet available in Loupe V(D)J Browser.
Cell Ranger 3.0 and later generates a .cloupe file for multi-species ("barnyard") experiments run with a joint reference. This allows Loupe Browser to compute an aggregate transcriptome expression count per cell, enabling easy segmentation by species, or joint analysis for host-virus applications. Please note that barnyard experiments are for 5’ single cell gene expression data only, not 5' single cell V(D)J.