Cell Ranger6.1, printed on 04/20/2024
The 5' Chromium Next GEM Single Cell Immune Profiling Solution with Feature Barcode technology enables the generation and analysis of gene expression, cell surface proteins (antibody capture), antigen specificity, and CRISPR guides in addition to V(D)J clonotypes. The antibody capture, antigen specificity and CRISPR guide RNA libraries are also referred to as Feature Barcode libraries.
Cell Ranger processes gene expression and Feature Barcode data from the 5' Single Cell Immune Profiling solution similar to the 3' Single Cell solution. The following pages take you to relevant sections on working with gene expression and Feature Barcode data. While these pages are hosted on 3' Single Cell solution's section, they are equally applicable to 5' Immune Profiling solution:
| Cell Ranger processes antigen specificity data similar to antibody capture data. Antigen specificity library construction and analysis in the absence of gene expression and antibody capture is not recommended. |