Cell Ranger ARC5.0, printed on 11/19/2024
Welcome to the Loupe Browser Multiome ATAC + Gene Expression tutorial. The next few pages will cover the features and functionality unique to data generated by Cell Ranger ARC and the Multiome ATAC + Gene Expression kit, and introduce techniques and patterns for analyzing linked gene expression and open chromatin regions.
This tutorial will not cover basic Loupe functionality for Gene Expression or ATAC data. To get more comfortable with analyzing Gene Expression and ATAC data, visit the tutorials for those data types:
The features covered in those tutorials will also apply to Single Cell Multiome ATAC + Gene Expression datasets generated by Cell Ranger ARC.
To use Loupe Browser, follow the directions on the Installation page to download and install the software on either macOS or Windows.
The Multiome ATAC + Gene Expression tutorial uses a 3k PBMC dataset that is not included with Loupe Browser. Click here to download the dataset (433MB).
Once the file is loaded, click here for a tour of multiome-specific features.
The tutorial dataset contains the results of a cellranger-arc run over a set of human peripheral blood mononuclear cells, with the standard Chromium™ Single-Cell Multiome ATAC + Gene Expression protocol.
The key metrics include:
This dataset has been pre-annotated with cell types derived from gene expression markers. See the 'Annotated Cell Types' Category, and 'Cell Type Markers' gene list preloaded with the dataset.
It should be noted that T-cell subtypes were more easily separable in ATAC projections, even though we used gene markers to define the subtypes.