10x Genomics
Chromium Single Cell Immune Profiling

Cell Ranger7.0, printed on 12/04/2024

What is Feature Barcode Technology for Immune Profiling?

Feature Barcode (FB) technology is a method for adding extra layers of information to cells by running Single Cell Gene Expression in parallel with other assays.

Feature Barcode technology compatible with the 5’ Chromium Next GEM Single Cell Immune Profiling assay

Antibody Capture (cell surface protein measurement): A method to label cell surface proteins using a specific protein binding molecule, such as an antibody conjugated to a Feature Barcode oligonucleotide (e.g. BioLegend TotalSeq™–C Antibodies).

Antigen Associated Capture: A method to rapidly map paired T and B cell receptor sequences to their corresponding antigens via the use of Multimeric MHC peptide complexes, such as dCODE™ Dextramer® reagents.

CRISPR: A method to analyze gene expression changes caused by the presence of CRISPR perturbations. The assay captures the full transcriptome and transfected guide RNAs from the same cell to correlate changes in the transcriptome to CRISPR perturbations.

10x Genomics provides an online Find Compatible Products tool to help customers obtain a comprehensive list of Feature Barcode compatible products and partners.

Analyzing and understanding Feature Barcode data

Visit the relevant pages for analyzing and understanding Feature Barcode data:

• For Feature Barcode terminology see the Feature Barcode Glossary.
• To run Cell Ranger on any combination of 5' single cell gene expression and Feature Barcode libraries refer to the Analyzing GEX/FB page.
• To learn how to view Feature Barcode data in Loupe Browser, see the Using Loupe Browser with Feature Barcode technology.
• For an additional tutorial on how to perform analysis with multiple library types (Single Cell V(D)J + 5′ Gene Expression + Feature Barcode technology), visit the Integrated Analysis section.
• To learn how to describe Feature Barcode reagents as inputs to the cellranger count pipeline, visit the Feature Barcode Reference page.