Cell Ranger7.1, printed on 12/10/2023
The 5' Chromium Next GEM Single Cell Immune Profiling Solution with Feature Barcode technology enables the analysis of gene expression, cell surface proteins (antibody), antigens, and CRISPR guides in addition to V(D)J clonotypes. The Antibody Capture, Antigen Capture, and CRISPR guide capture libraries are also referred to as Feature Barcode libraries.
Cell Ranger processes Gene Expression and Feature Barcode data from the 5' Single Cell Immune Profiling solution similar to the 3' Single Cell solution. The following pages take you to relevant sections on working with Gene Expression and Feature Barcode data. While some of these pages are hosted on the 3' Single Cell solution's section, they are equally applicable to the 5' Immune Profiling solution:
|Cell Ranger processes Antigen Associated Capture data similar to Antibody Capture data. Antigen Associated Capture library construction and analysis in the absence of Gene Expression and Antibody Capture is not recommended.|