Cell Ranger2.0, printed on 07/04/2020
You can save your dataset workspace, including the custom groups you've created and any gene lists you've created or imported, by clicking on the Save (disk) icon on the toolbar. If you prefer to create a new version of a .cloupe file, choose "Save As" from the File menu, or press Ctrl-Shift-S (Windows) or Command-Shift-S (Mac). You'll be prompted to create a new .cloupe file somewhere in your file system.
Loupe Cell Browser files are self contained, so if you want to share a dataset with a colleague, you can send him or her the .cloupe file.
In Loupe Cell Browser 1.0.5 and later, you can both import and export categories. This allows you to integrate data from other pipelines or scripts into Loupe Cell Browser, or export manually assigned categories into custom scripts. For more information on importing and exporting categories, consult the v1.0.5 release notes.
You may also currently export four items from Loupe Cell Browser:
To export the tabular list of significant genes, including p-values and log2 fold changes, click on the Export icon above the tabular view in the data panel. This will save the table in CSV format. When heatmap mode is selected, click on the camera icon to export the heatmap as a .PNG. Example output is shown below (right-click to save and view at higher resolution):
To export the lists of genes you have created in the Gene Expression sidebar, click on the three dots (More Options) button atop the sidebar and select 'Export Lists' from the popup menu. You can import these lists into another dataset to quickly identify cell subtypes.
Finally, to take a screenshot of the plot, click on the camera button in the toolbox. You may want to minimize the sidebar and data panel by clicking their arrow buttons, and then recenter the plot to show it aligned and in highest detail.
This concludes the Loupe Cell Browser gene expression tutorial. Now it's time to use what you've learned on your own data. Read about how to generate your own .cloupe files with the Cell Ranger pipeline.
If you have both gene expression and immune repertoire data from the same dataset, or you're interested in the V(D)J analysis capabilities of Loupe Cell Browser 2.0, proceed to Integrated Gene Expression and V(D)J Analysis in Loupe Cell Browser.
If you encounter any errors in the program, you can send a bug report at any time by clicking on "Send Bug Report" from the Help menu. A .tar.gz file containing logs from your most recent Loupe Cell Browser session will be created, and you can send that file to [email protected]. Please add the subject line "Loupe Cell Browser Error" to your message.
You may also submit general feedback and feature requests to [email protected] as well.
We hope this tutorial has made you familiar with the capabilities of Loupe Cell Browser, and made you excited to process your own data. We hope you find it to be the easiest, fastest and most enjoyable way to interpret your single-cell gene expression data.