Space Ranger1.2, printed on 10/13/2024
The pipeline output contains a subdirectory named spatial
which stores imaging related files. The files include the
following:
tissue_hires_image.png
and tissue_lowres_image.png
: These files are downsampled versions of the original,
full-resolution brightfield image provided by the user. Downsampling is accomplished by box filtering, which averages
RGB values in patches of pixels in the full-resolution image to obtain an RGB value of one pixel in the downsampled
image. The tissue_hires_image.png
image has 2,000 pixels in its largest dimension, and the tissue_lowres_image.png
has 600 pixels.
aligned_fiducials.jpg
: This image has the dimensions of tissue_hires_image.png
. Fiducial spots found by the
fiducial alignment algorithm are highlighted in red. This file is useful to verify that fiducial alignment was
successful.
scalefactors_json.json
: This file contains the following fields:
tissue_hires_scalef
: A scaling factor that converts pixel positions in the original, full-resolution image to
pixel positions in tissue_hires_image.png
.tissue_lowres_scalef
: A scaling factor that converts pixel positions in the original, full-resolution image to
pixel positions in tissue_lowres_image.png
.fiducial_diameter_fullres
: The number of pixels that span the diameter of a fiducial spot in the original,
full-resolution image.spot_diameter_fullres
: The number of pixels that span the diameter of a tissue spot in the original,
full-resolution image.$ cd /home/jdoe/runs/sample345/outs/spatial $ cat scalefactors_json.json {"spot_diameter_fullres": 89.45248682925602, "tissue_hires_scalef": 0.17699115, "fiducial_diameter_fullres": 144.5001710318751, "tissue_lowres_scalef": 0.053097345}
detected_tissue_image.jpg
: This image has the dimensions of the tissue_hires_image.png
and shows the following:
tissue_positions_list.csv
: This text file contains a table with rows that correspond to spots. It has 4,992 rows,
which is the number of spots in the spatial array. Columns, whose names are not specified in the file, correspond to the
following fields:
barcode
: The sequence of the barcode associated to the spot.in_tissue
: Binary, indicating if the spot falls inside (1) or outside (0) of tissue.array_row
: The row coordinate of the spot in the array from 0 to 77. The array has 78 rows.array_col
: The column coordinate of the spot in the array. In order to express the orange crate arrangement of
the spots, this column index uses even numbers from 0 to 126 for even rows, and odd numbers from 1 to 127 for odd
rows. Notice then that each row (even or odd) has 64 spots.pxl_row_in_fullres
: The row pixel coordinate of the center of the spot in the full resolution image.pxl_col_in_fullres
: The column pixel coordinate of the center of the spot in the full resolution image.$ cd /home/jdoe/runs/sample345/outs/spatial $ head -2 tissue_positions_list.csv ACGCCTGACACGCGCT-1,0,0,0,910,1261 TACCGATCCAACACTT-1,0,1,1,1030,1329