Cell Ranger DNA, printed on 12/11/2024
Analysis software for the 10x Genomics single cell DNA product is no longer supported. Raw data processing pipelines and visualization tools are available for download and can be used for analyzing legacy data from 10x Genomics kits in accordance with our end user licensing agreement without support. |
Cell Ranger DNA pipelines are distributed for and run on 64-bit Linux systems. The runtime and storage requirements of Cell Ranger DNA scale linearly with the number of reads to be processed. In the recommended experiment (1000 cells at 750,000 read-pairs per cell) there will be 750 million read-pairs and the minimum system requirements are:
The pipelines also run on clusters that meet these additional minimum requirements:
Most software dependencies come bundled in the Cell Ranger DNA package. However, cellranger-dna mkfastq
also requires:
Limit | Recommendation |
---|---|
user open files | 16k |
system max files | 10k per GB RAM available to Cell Ranger DNA |
user processes | 64 per core available to Cell Ranger DNA |
The performance data below was produced by running Cell Ranger DNA on a range of datasets and computers. Cell Ranger DNA runtime is almost entirely determined by the number of reads and is insensitive to the number of cells. The table below was created on hardware available from Amazon cloud services.
Number of read-pairs | Small system | Medium system | Large system |
---|---|---|---|
375 million | 12h | 6h | 4h |
1.2 billion | 25h | 13h | 11h |
7 billion | Not recommended | Not recommended | 114h |
CPU cores | RAM (GB) | CPU Generation | Disk | |
---|---|---|---|---|
Small system | 16 | 128 | Ivy Bridge | HDD |
Medium system | 32 | 256 | Broadwell | HDD |
Large system | 48 | 384 | Skylake | SDD |