Single Cell Gene Expression Demonstrated Protocol Compatibility Table

Demonstrated Protocol, Last Modified on November 19, 2018, Permalink

Protocol SC3'v2 SC3'v3 SC3'v3 with CRISPR Screening SC3'v3 with Cell Surface Protein
Single Cell Protocols - Cell Preparation Guide
Methanol Fixation of Cells for Single Cell RNA Sequencing ✓*
Isolation of Nuclei for Single Cell RNA Sequencing ✓* -
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling ✓* ✓* -
Tumor Dissociation for Single Cell RNA Sequencing ✓* -
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing ✓* ✓* ✓*
Moss Protoplast Suspension for Single Cell RNA Sequencing ✓*
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing ✓*
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing ✓* ✓*
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols ** -

✓ Compatible (tested in-house)
✓* Untested but low risk
** Compatible but unsupported
- Untested, use at your own risk
✘ Incompatible