Single Cell Gene Expression Demonstrated Protocol Compatibility Table

Demonstrated Protocol, Last Modified on December 2, 2021, Permalink

Protocol	SC3'v3/v3.1	SC3'v3/v3.1 with CRISPR Screening	SC3'v3/v3.1 with Cell Surface Protein	SC3’v3.1 with Cell Multiplexing
Single Cell Protocols - Cell Preparation Guide	✓	✓	✓	✓
Methanol Fixation of Cells for Single Cell RNA Sequencing	✓*	✘	✘	✘
Isolation of Nuclei for Single Cell RNA Sequencing & Tissues for Single Cell RNA Sequencing	✓*	✘	-	✓
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling	✓*	-	✓*	✓*
Tumor Dissociation for Single Cell RNA Sequencing	✓*	-	✓	✓*
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing	✓	✓	✓	✓
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing	✓*	✓*	✓*	✓*
Moss Protoplast Suspension for Single Cell RNA Sequencing	✓*	✘	✘	-
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing	✓	✓	✓	-
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing	✓	-	✓	✓
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing	✓	-	✓*	✓
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols	✓	-	✓	-
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing	✓	-	✓*	✓
Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols	-	✓	✓	✓

✓ Compatible (tested in-house)
✓* Untested but low risk
** Compatible but unsupported
- Untested, use at your own risk
✘ Incompatible