10x Genomics Support/Single Cell Gene Expression/Documentation/Sample Prep/

Single Cell Gene Expression Demonstrated Protocol Compatibility Table

ProtocolSC3'v3/v3.1SC3'v3/v3.1 with CRISPR ScreeningSC3'v3/v3.1 with Cell Surface ProteinSC3’v3.1 with Cell Multiplexing
Single Cell Protocols - Cell Preparation Guide✓✓✓✓
Isolation of Nuclei for Single Cell RNA Sequencing & Tissues for Single Cell RNA Sequencing✓*✘-✓
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling✓*-✓*✓*
Tumor Dissociation for Single Cell RNA Sequencing✓*-✓✓*
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing✓✓✓✓
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing✓*✓*✓*✓*
Moss Protoplast Suspension for Single Cell RNA Sequencing✓*✘✘-
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing✓✓✓-
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing✓-✓✓
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing✓-✓*✓
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols✓-✓-
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing✓-✓*✓
Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols-✓✓✓

✓ Compatible (tested in-house) ✓* Untested but low risk ** Compatible but unsupported - Untested, use at your own risk ✘ Incompatible

Document Type
Demonstrated Protocol
Last Modified
June 17, 2022