Single Cell Gene Expression Demonstrated Protocol Compatibility Table

Demonstrated Protocol, Last Modified on December 2, 2021, Permalink

Protocol SC3'v3/v3.1 SC3'v3/v3.1 with CRISPR Screening SC3'v3/v3.1 with Cell Surface Protein SC3’v3.1 with Cell Multiplexing
Single Cell Protocols - Cell Preparation Guide
Methanol Fixation of Cells for Single Cell RNA Sequencing ✓*
Isolation of Nuclei for Single Cell RNA Sequencing & Tissues for Single Cell RNA Sequencing ✓* -
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling ✓* - ✓* ✓*
Tumor Dissociation for Single Cell RNA Sequencing ✓* - ✓*
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing ✓* ✓* ✓* ✓*
Moss Protoplast Suspension for Single Cell RNA Sequencing ✓* -
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing -
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing -
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing - ✓*
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols - -
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing - ✓*
Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols -

✓ Compatible (tested in-house)
✓* Untested but low risk
** Compatible but unsupported
- Untested, use at your own risk
✘ Incompatible