Moss Protoplast Suspension for Single Cell RNA Sequencing

Demonstrated Protocol, Last Modified on June 29, 2017, Permalink

CG000090_SamplePrepDemonstratedProtocol - Moss Protoplasts_RevC.pdf

This Demonstrated Protocol outlines how to obtain a single protoplast suspension from the spreading earthmoss, Physcomitrella patens, (Grandsen or Villesexel ecotypes) in preparation for use in 10x Genomics® Single Cell Protocols. The protoplasts are “naked” plant cells without the cell wall but with an intact plasma membrane.