Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling

Demonstrated Protocol, Last Modified on July 31, 2017, Permalink

CG000123_SamplePrepDemonstratedProtocol - CD3+ Enrichment_RevB.pdf

This Demonstrated Protocol outlines best practices for enriching the percentage of Cluster of Differentiation 3 positive (CD3+) T cells obtained from dissociated tumors in preparation for use in 10x Genomics® Single Cell Protocols.

While this Protocol is demonstrated with tissue matched dissociated primary tumor cells and peripheral blood mononuclear cells (PBMCs) from a clear cell renal carcinoma (CCRC) patient, it has also been demonstrated with dissociated primary tumor cells from colorectal cancer (CRC) and renal cell carcinoma (RCC) patients, and with lymph node cells from melanoma patients. This Protocol may also be used as a basis for enrichment of the CD3+ T cell population from other dissociated tissues as well as other primary cells in preparation for use in 10x Genomics® Single Cell Protocols. Modifications to this Protocol may be necessary for other sample types (e.g. media type, resuspension buffer, centrifugation speed and time).