Demonstrated Protocol, Last Modified on March 31, 2021, Permalink
This protocol outlines thawing of frozen dissociated tumor cells for use with 10x Genomics Single Cell protocols. The protocol was demonstrated using dissociated tumor cells from glioblastoma, renal cell carcinoma, and endometrial cancer patients.
While this Demonstrated Protocol is specific to dissociated tumor cells, the protocol may be used as a basis for handling other primary/fragile cells in preparation for use in the 10x Genomics Single Cell protocols.
This demonstrated protocol is optimized for counting cells in the range of 700-1200 cells/µl. If using the Single Cell 3' LT v3.1 (low throughput) application, ensure cells are counted as indicated in this protocol and then diluted to the LT specific optimal loading concentration of 100-600 cells/µl using the Cell Dilution Overview in the LT User Guide.