Single Cell Gene Expression LT Demonstrated Protocol Compatibility Table

Demonstrated Protocol, Last Modified on August 19, 2021, Permalink

Protocol SC3'LTv3.1 SC3'LTv3.1 with Cell Surface Protein
Single Cell Protocols - Cell Preparation Guide
Methanol Fixation of Cells for Single Cell RNA Sequencing -
Isolation of Nuclei for Single Cell RNA Sequencing ✓* -
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling ✓* -
Tumor Dissociation for Single Cell RNA Sequencing ✓* ✓*
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing ✓*
Moss Protoplast Suspension for Single Cell RNA Sequencing -
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing -
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing ✓*
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing ✓*
Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols ** **

✓ Compatible (tested in-house)
✓* Untested but low risk
** Compatible but unsupported
- Untested, use at your own risk
✘ Incompatible