Chromium De Novo Assembly
Supernova1.2, printed on 01/21/2022
Supernova pipelines run on individual Linux systems that meet these minimum requirements:
- 16-core (or greater) Intel or AMD processor
- 244 GB RAM for a human at 56x coverage
- No other large processes running on the system, except when all processes have their memory capped appropriately. Supernova has an option (described in Assembly Process) for capping its memory usage. Running multiple large user processes on the same server will have an unpredictable effect on run time.
- 2 TB free disk space
- 64-bit CentOS/RedHat 5.2+ or Ubuntu 8+
In addition, Supernova must be run on a system with the following software pre-installed:
bcl2fastq 2.17 or higher is preferred and supports most sequencers running RTA version 1.18.54 or higher. If you are using a NovaSeq, please use version 2.20 or higher. If your sequencer is running an older version of RTA, then bcl2fastq 1.8.4 is required by Illumina.