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10x Genomics
Chromium De Novo Assembly

Overview of De Novo Assembly Software

Supernova is a software package for de novo assembly from Chromium Linked-Reads that are made from a single whole-genome library from an individual DNA source. A key feature of Supernova is that it creates diploid assemblies, thus separately representing maternal and paternal chromosomes over very long distances. Almost all other methods instead merge homologous chromosomes into single incorrect 'consensus' sequences. Supernova is the only practical method for creating diploid assemblies of large genomes.

The Supernova software package includes two processing pipelines:

and for post-processing:

More information

Supernova is tested and validated on human germline data. Please see the Supernova guidance for non-human considerations.

See the manuscript "Direct determination of diploid genome sequences" for broad algorithmic details and assessment of performance and assembly quality for Supernova.