Single Cell V(D)J Demonstrated Protocol Compatibility Table

Demonstrated Protocol, Last Modified on May 20, 2020, Permalink

Protocol	SC5' V(D)J	SC5' V(D)J with Feature Barcoding technology
Single Cell Protocols - Cell Preparation Guide	✓	✓
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling	✓	✓*
Tumor Dissociation for Single Cell RNA Sequencing	✓*	✓*
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing	✓	✓*
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing	✓	✓
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing	✓	✓*
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing	✓	✓
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing	✓	✓
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols	✘	✓
Cell Labeling with Dextramers for Single Cell RNA Sequencing Protocols*	✘	✓
Methanol Fixation of Cells for Single Cell RNA Sequencing	✘*	✘
Isolation of Nuclei for Single Cell RNA Sequencing	✘*	✘

✓ Compatible (tested in-house)
✓* Untested but low risk
✘* Untested but high risk
✘ Not compatible
*Antigen-only analysis is not currently supported by 10x Genomics. For antigen analysis, we recommend staining with dextramer reagents in conjunction with a small panel of TotalSeq C antibodies. This increases the cell calling accuracy of the data. Please contact Tech Support for TotalSeq C antibody recommendations.