Single Cell V(D)J Demonstrated Protocol Compatibility Table

Demonstrated Protocol, Last Modified on December 4, 2019, Permalink

Protocol	SC5' V(D)J	SC5' V(D)J with Feature Barcoding technology
Single Cell Protocols - Cell Preparation Guide	✓	✓
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling	✓	✓*
Tumor Dissociation for Single Cell RNA Sequencing	✓*	✓*
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing	✓	✓
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing	✓	✓*
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing	✓	✓
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing	✓	✓
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols	✘	✓
Cell Labeling with Dextramers for Single Cell RNA Sequencing Protocols	✘	✓
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing	✓	✓*

✓ Compatible (tested in-house)
✓* Untested but low risk
✘ Incompatible