Tumor Dissociation for Single Cell RNA Sequencing

Demonstrated Protocol, Last Modified on May 22, 2020, Permalink


This Demonstrated Protocol outlines best practices for dissociation of mouse melanoma, colon tumor, and breast tumor for use in 10x GenomicsĀ® Single Cell protocols.

While this protocol is demonstrated with freshly obtained (not frozen) mouse melanoma, colon tumor, and breast tumor, it may be used for dissociating other tumor types for use in 10x Genomics Single Cell protocols. Modifications to this demonstrated protocol may be necessary for other tumor types (e.g. dissociation time, resuspension buffer, enzyme concentration, centrifugation speed and time).