Long Ranger1.3, printed on 12/26/2024
The Long Ranger software strives to maintain compatibility with common analysis tools by using standard output file formats whenever possible. For example, the barcoded BAM files can be viewed in standard genome browsers such as IGV to verify alignment quality and other features. The GemCode-specific data, including barcodes and haplotyping, can be accessed via any third-party tools or scripts that can parse the additional elements utilized by Long Ranger.
All pipelines produce all of their output in a single pipeline output directory which, by default, is named according to the flowcell serial number for longranger demux (e.g., HAWT7ADXX) and --id name for longranger run. Output files will appear in the outs/ subdirectory within this pipeline output directory. For example, a typical Exome Mode run may look like:
$ cd /home/jdoe/runs $ longranger run --id=sample345 \ --sex=female \ --fastqs=/home/jdoe/runs/HAWT7ADXX/outs/fastq_path \ --reference=/opt/refdata-hg19-1.2.0 \ --indices=SI-001 \ --targets=/home/jdoe/runs/agilent_exome.bed Martian Runtime - 1.3.1 Running preflight checks (please wait)... 2012-01-01 12:00:00 [runtime] (ready) ID.sample345.PHASER_SVCALLER_CS.PHASER_SVCALLER._ALIGNER.SETUP_CHUNKS ... 2015-01-02 15:46:44 [runtime] (join_complete) ID.sample345.PHASER_SVCALLER_CS.PHASER_SVCALLER.LOUPE_PREPROCESS 2015-01-02 15:46:55 [runtime] VDR killed 4738 files, 223GB. Outputs: - Run summary: /home/jdoe/runs/sample345/outs/summary.csv - BAM barcoded: /home/jdoe/runs/sample345/outs/phased_possorted_bam.bam - BAM index: /home/jdoe/runs/sample345/outs/phased_possorted_bam_index.bam.bai - VCF phased: /home/jdoe/runs/sample345/outs/phased_variants.vcf.gz - VCF index: /home/jdoe/runs/sample345/outs/phased_variants_index.vcf.gz.tbi - SV calls: /home/jdoe/runs/sample345/outs/sv_calls.bedpe - SV candidates: /home/jdoe/runs/sample345/outs/sv_candidates.bedpe - SV phasing: /home/jdoe/runs/sample345/outs/sv_phasing.tsv - Loupe file: /home/jdoe/runs/sample345/outs/loupe.loupe Pipestance completed successfully!
In this case,
/home/jdoe/runs/ is where the pipeline was run
/home/jdoe/runs/sample534/ is the pipeline output directory
/home/jdoe/runs/sample534/outs/ contains the pipeline output files
The contents of this outs/ directory contain the data that is described in the remainder of this section: barcoded and phased BAMs, VCFs and SV BEDPEs.
More information about the contents of the pipeline output directory can be found in the Pipestance Structure page.