Supernova1.0, printed on 10/26/2020
Supernova generates highly-contiguous, phased, whole-genome de novo assemblies from a Chromium-prepared library.
|Supernova should be run with at most 1.2 billion reads, and at 38-56x coverage of the genome. Please see Sample Requirements and System Requirements before creating your Chromium libraries for assembly.|
This involves the following steps:
Run supernova demux on the Illumina BCL output folder to generate FASTQ files.
Run supernova run separately for each sample that was demultiplexed by supernova demux to generate a whole genome de novo assembly.
Run supernova mkfasta in order to generate various styles of FASTA output for your assemblies.
For the following example, assume that the Illumina BCL output is in a folder named
First, follow the instructions on running supernova demux to generate FASTQ files. For example, if the flowcell serial number was
HAWT7ADXX, then supernova demux will output FASTQ files in
To run Supernova, you use the supernova run command, with the following parameters:
|A unique run ID string: e.g. |
|Path of the FASTQ folder generated by supernova demux|
|(optional) Description of the data set. This will be included, along with the run ID string, in various output files.|
|(optional) Sample name as specified in the sample sheet supplied to |
|(optional) Sample indices associated with this sample. Comma-separated list of:
|(optional) Lanes associated with this sample|
|(optional) Specify number of reads to downsample to, if a surplus of data is available.|
After determining these input arguments, call supernova run:
$ cd /home/jdoe/runs $ supernova run --id=sample345 \ --fastqs=/home/jdoe/runs/HAWT7ADXX/outs/fastq_path \ --indices=SI-GA-A1
Following a set of preflight checks to validate input arguments, Supernova pipeline stages will begin to run:
supernova run Copyright (c) 2016 10x Genomics, Inc. All rights reserved. ----------------------------------------------------------------------------- Martian Runtime - 2.0.0 Running preflight checks (please wait)... 2016-01-01 00:00:01 [runtime] (ready) ID.sample345.ASSEMBLER_CS._ASSEMBLER_PREP.SETUP_CHUNKS 2016-01-01 00:00:01 [runtime] (split_complete) ID.sample345.ASSEMBLER_CS._ASSEMBLER_PREP.SETUP_CHUNKS ...
As a default, supernova run will use all of the sequence data available in the FASTQ folder with the specified
--lanes. If you would like to downsample the data you can optionally specify the number of reads that Supernova should assemble using the
--reads. By default, supernova run will use all of the cores available on your system to execute pipeline stages.
The pipeline will create a new folder named with the sample ID you specified (e.g.
/home/jdoe/runs/sample345) for its output. If this folder already exists, supernova run will assume it is an existing pipestance and attempt to resume running it.
A successful supernova run execution should conclude with a message that looks similar to this:
... 2016-01-03 00:00:01 [runtime] (chunks_complete) ID.sample345.ASSEMBLER_CS._ASSEMBLER_CP 2016-01-03 00:00:01 [runtime] (run:local) ID.sample345.ASSEMBLER_CS._ASSEMBLER_CP.fork0.join 2016-01-03 00:00:03 [runtime] (join_complete) ID.sample345.ASSEMBLER_CS._ASSEMBLER_CP Outputs: - assembly: /home/jdoe/runs/sample345/outs/assembly - summary: /home/jdoe/runs/sample345/outs/summary.csv - report: /home/jdoe/runs/sample345/outs/report.txt Pipestance completed successfully! Saving pipestance info to sample345/sample345.mri.tgz
The output of the pipeline will be contained in a folder named with the sample ID you specified (e.g.
sample345). The subfolder named
outs will contain
the main pipeline output files:
|Run summary metrics in CSV format|
|Extensive assembly metrics in human-readable form|
|The directory containing the assembly in binary format|
First, familiarize yourself with the representation of a genome assembly as a graph structure. Next, follow the instructions on running supernova mkfasta to generate FASTA files.