HMW DNA QC

Demonstrated Protocol, Last Modified on July 7, 2017, Permalink

CG00019_SamplePrepDemonstratedProtocol_-_DNAQC_RevB.pdf

The Chromium Genome Protocols generate long-range information across the length of individual DNA molecules. Starting the process with High Molecular Weight (HMW) genomic DNA (gDNA) will typically result in better application performance, such as increased haplotype phase block length and ability to call structural variants. Optimal performance has been characterized on input gDNA with a mean length >50 kb.

However, gDNA samples may exist that do not meet this specification, for example:

• gDNA samples may not have been extracted using an optimized HMW extraction protocol
• gDNA samples may be old and the DNA has degraded
• HMW gDNA isolation is generally difficult for certain sample types (e.g. solid tissue)

Due to these variables, input gDNA quality control (QC) is highly recommended to maximize Linked-Read application performance.