QC of Chromium Genome Libraries: Quantitative Evaluation Using qPCR

Technical Note, v1 Chemistry, Last Modified on November 19, 2018, Permalink

CG00048_10x_Technical Note_Library_Quantity.pdf

Technical Note - Quantitative Evaluation of Genome Libraries using qPCR

This Technical Note discusses qPCR quantification of Chromium Genome Libraries for evaluating the success of library generation and providing accurate quantitative information. While Bioanalyzer traces are used for qualitative assessment of libraries, KAPA Library Quantification qPCR is the method of choice for quantitative measurements due to its sensitivity in measuring low DNA concentrations and its specificity for PCR-competent DNA molecules. It is crucial to accurately quantify Chromium™ Genome Libraries for optimal sample loading, clonal amplification and cluster density on an Illumina® flowcell.