Sample Prep

10x Genomics Sample Preparation Products

Fixed RNA Profiling

• Fixation of Cells & Nuclei for Chromium Fixed RNA Profiling
• Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling

Chromium Nuclei Isolation Kit

• Chromium Nuclei Isolation Kit Sample Prep User Guide
• Chromium Nuclei Isolation Kit Tested Tissues
• Chromium Nuclei Isolation Kit How-to Videos

Demonstrated Protocol

• Single Cell Protocols - Cell Preparation Guide
• Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
• Isolation of Nuclei for Single Cell RNA Sequencing & Tissues for Single Cell RNA Sequencing
• Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing
• Tumor Dissociation for Single Cell RNA Sequencing
• Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing
• Isolation of Leukocytes, Bone Marrow and Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing

Fixed RNA Profiling

• Cell Surface Protein Labeling for Chromium Fixed RNA Profiling for Singleplexed Samples with Feature Barcode technology

Technical Note

• Pipette Tip Recommendations for 10x Genomics Single Cell Protocols
• Performance of Blood Collection Tubes and PBMC Isolation Methods for Single Cell Gene Expression

Feature Barcoding technology

• Quality Control of Cell Surface Protein Labeling using Flow Cytometry

SDS

• Chromium Nuclei Isolation Kit Safety Data Sheets