High Input Genomic DNA Length Results in Optimal Performance of the Chromiumâ„¢ Genome Protocols

Demonstrated Protocol, Last Modified on July 7, 2017, Permalink


The Chromium Genome Protocols generate long-range information across the length of individual DNA molecules. Starting the process with High Molecular Weight (HMW) genomic DNA (gDNA) will typically result in better application performance, such as increased haplotype phase block length and ability to call structural variants. Optimal performance has been characterized on input gDNA with a mean length >50 kb.

However, gDNA samples may exist that do not meet this specification, for example:

Due to these variables, input gDNA quality control (QC) is highly recommended to maximize Linked-Read application performance.