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10x Genomics
Targeted Gene Expression

Sequencing Targeted Single Cell Gene Expression libraries

For sequencing Targeted Single Cell Gene Expression libraries, use the same sequencing parameters recommended for their parent Single Cell Gene Expression or Single Cell Immune Profiling libraries.

Supported Sequencers

Sequencing Depth

The minimum recommended sequencing depth is 2,000 read pairs per cell for pre-designed panels. When using Custom Panels, optimization of sequencing depth may be required based on panel size and expression levels of panel genes. The Cell Ranger targeted-depth tool can recommend sequencing depth for a Targeted Gene Expression library enriched from an existing whole transcriptome library analyzed with Cell Ranger (or enriched from a similar library).