Sequencing Metrics & Base Composition of Visium Spatial Gene Expression Libraries

Technical Note, Last Modified on October 27, 2020, Permalink


The Visium Spatial Gene Expression Reagent Kits workflow produces Visium Spatial Gene Expression libraries for measuring total mRNA in intact tissue sections. This Technical Note presents a comparison of sequencing metrics for pooled Visium Spatial Gene Expression libraries across Illumina️ platforms. The expected base percentage profiles and Phred quality scores based on a control library are described to provide general guidance on the expected range of sequencing metrics on Illumina platforms. Individual results may vary depending on the specific sequencing instrument and/or particular sample and loading characteristics.