Sequencing Metrics & Base Composition of Visium Spatial Gene Expression Libraries

Technical Note, Last Modified on October 27, 2020, Permalink

CG000290_TechNote_SequencingMetrics&BaseCompofVisiumSpatialGeneExpressionLibraries_RevA.pdf

The Visium Spatial Gene Expression Reagent Kits workflow produces Visium Spatial Gene Expression libraries for measuring total mRNA in intact tissue sections. This Technical Note presents a comparison of sequencing metrics for pooled Visium Spatial Gene Expression libraries across Illumina️ platforms. The expected base percentage profiles and Phred quality scores based on a control library are described to provide general guidance on the expected range of sequencing metrics on Illumina platforms. Individual results may vary depending on the specific sequencing instrument and/or particular sample and loading characteristics.

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