Methanol Fixation, H&E Staining & Imaging for Visium Spatial Protocols

Demonstrated Protocol, Last Modified on August 10, 2021, Permalink


The Visium Spatial Gene Expression Solution measures the total mRNA in tissue sections and requires a Visium Spatial slide with intact tissue sections as input. This protocol outlines methanol fixation, Hematoxylin & Eosin (H&E) staining, and imaging of tissue for use with 10x Genomics Visium Spatial protocols. Fixed and stained tissue sections are inputs for the downstream Visium Spatial Tissue Optimization and Visium Spatial Gene Expression workflows.