Visium Spatial Gene Expression Imaging Guidelines

Technical Note, Last Modified on April 1, 2021, Permalink


The Visium Spatial Gene Expression Solution measures total mRNA in intact tissue sections and maps where gene activity is occurring. Successful gene expression visualization is highly dependent on good imaging practices. This Technical Note provides hardware recommendations, general image acquisition and analysis guidelines, and examples of images that are suitable for downstream analysis. Individual results may vary depending on the specific imaging system, and/or sample characteristics. Consult the Visium Spatial Gene Expression Reagent Kits Library Preparation User Guide (CG000239) and the Visium Spatial Gene Expression Reagent Kits – Tissue Optimization User Guide (CG000238) for complete protocols.