Visium Spatial Gene Expression for FFPE – Tissue Preparation Guide

Demonstrated Protocol, Last Modified on June 9, 2021, Permalink

CG000408_Demonstrated_Protocol_VisiumSpatialProtocolsFFPE_TissuePreparationGuide_RevA.pdf

The Visium Spatial Gene Expression for FFPE assays mRNA levels by using probes against the whole transcriptome in tissue sections derived from formalin fixed & paraffin embedded (FFPE) tissue samples and requires a Visium Spatial slide with intact sections as input. Proper tissue handling and preparation techniques preserve the morphological quality of the tissue sections and the integrity of mRNA transcripts. Maintaining tissue adhesion and high-quality RNA is critical to assay performance.

This protocol is only compatible with Spatial Gene Expression for FFPE reagent kits. Do NOT use with the Visium assay for snap frozen and OCT embedded tissue.