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10x Genomics
Chromium Single Cell Immune Profiling

Analysis of Multiple Library Types

Single Cell V(D)J + 5′ Gene Expression + Feature Barcode Technology for Cell Surface Protein Analysis

The Chromium Single Cell Immune Profiling Solution with Feature Barcode technology enables analysis of V(D)J clonotypes, gene expression, cell surface protein, and antigen specificity (Feature Barcode) simultaneously, in the same single cell.

Analysis of each of the sequenced libraries, alone or in combination with other library types, can be performed using the latest versions of Cell Ranger, Loupe Browser, and Loupe V(D)J Browser.

Running Cell Ranger

The following illustration depicts how the cellranger multi pipeline can be used to analyze FASTQ data derived from the three Immune Profiling library types from the same GEM Well:

The pipeline will generate a Loupe V(D)J Browser .vloupe file for each of the V(D)J T cell and B cell enriched libraries, in addition to a variety of files to describe V(D)J clonotypes, consensus sequences, and contigs. The pipeline will also generate a Loupe Browser .cloupe file, a feature-barcode matrix, and other information from the gene expression and Feature Barcode library. For more details on running the V(D)J analysis pipeline, consult the Cell Ranger multi documentation.

Loupe Tutorials

The Loupe Browser and Loupe V(D)J Browser allow you to tie gene expression, cell surface protein and V(D)J information from the pipeline run, back together.

To learn more about how to conduct multimodal analysis of V(D)J and gene expression data from the same sample, start the Loupe V(D)J + Gene Expression Tutorial. In the tutorial, you will be able to download example 5′ gene expression profiles and T cell clonotypes from a non-small cell lung carcinoma, and use the Loupe browsers to analyze the integrated data.

To learn more about analyzing Feature Barcode data, start the Immune Profile Analysis with Feature Barcode and Surface Marker Expression tutorial. In this tutorial, you will download and explore 5′ gene expression and Cell Surface Protein data from a PBMC sample with Loupe Browser.