Cell Labeling with Dextramers for Single Cell RNA Sequencing Protocols

Demonstrated Protocol, Last Modified on September 9, 2019, Permalink


20190828_CG000203 DextramerLabeling_RevAtoRevB.pdf

Multimeric MHC peptide complexes, such as Dextramers, bind to T-cell receptors (TCRs) with high affinity, which can enable detection of TCR antigen specificity. This protocol provides guidance for labeling cells with dCODE Dextramer (Dextramer MHC-Feature Barcode oligonucleotide conjugate), along with guidance for enriching Dextramer+ T cells by Fluorescence Activated Cell Sorting (FACS). These Dextramer labeled cells can be used for generating Chromium Single Cell libraries as described in the User Guide for Chromium Immune Profiling Solution with Feature Barcoding technology (CG000186, CG000208).