Sample Prep

Demonstrated Protocol (12 documents)

Sample Prep

• Isolation of Leukocytes, Bone Marrow and Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing
• Single Cell V(D)J Demonstrated Protocol Compatibility Table
• Single Cell Protocols - Cell Preparation Guide
• Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols
• Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
• Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling
• Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing
• Tumor Dissociation for Single Cell RNA Sequencing
• Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing
• Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing
• Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing
• Cell Labeling with Dextramers for Single Cell RNA Sequencing Protocols

Technical Note

Feature Barcoding technology

• Quality Control of Cell Surface Protein Labeling using Flow Cytometry

Gene Expression

• Resolving Cell Types as a Function of Read Depth and Cell Number
• Removal of Dead Cells from Single Cell Suspensions Improves Performance for 10x Genomics® Single Cell Applications
• Importance of Cell Stock Concentration for Accurate Target Cell Recovery
• Guidelines for Accurate Target Cell Counts Using 10x Genomics® Single Cell Solutions
• Chromium Single Cell Applications - Guidelines for Optimal Sample Preparation