Getting Started: Single Cell Immune Profiling

Getting Started, Last Modified on November 9, 2020, Permalink


The Chromium Single Cell Immune Profiling Solution provides a scalable microfluidic platform for simultaneous generation of full-length, paired V(D)J sequences, as well as gene expression, cell surface protein expression, and antigen specificity data at a single cell level. It is done by partitioning thousands of cells into nanoliter-scale Gel Beads-in-emulsion (GEMs), where all generated cDNA molecules from an individual GEM share a common 10x Barcode. Sequencing-ready libraries are generated and sequenced, and the unique 10x barcodes are used to associate reads back to the individual partitions. This document provides guidance on how to get started with the Single Cell Immune Profiling assay.