Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols

Demonstrated Protocol, Last Modified on September 9, 2019, Permalink

CG000149_DemonstratedProtocol_CellSurfaceProteinLabeling_RevB.pdf

20190828_CG000149 Cell_SurfaceProteinLabeling_RevAtoRevB.pdf

CG000193_Barcode_Whitelist_forCustom_Feature_Barcoding conjugates_RevA.txt  (right-click and Save Link As...)
CG000193 Barcode Whitelist forCustom Feature Barcoding conjugates RevA

Cell surface proteins can be labeled using a specific protein binding molecule, such as an antibody conjugated to a Feature Barcode oligonucleotide. This protocol provides guidance for antibody-oligonucleotide conjugation and outlines cell surface protein labeling for use with:

• Chromium Single Cell 3' Reagent Kits v3 User Guide with Feature Barcoding technology for Cell Surface Protein (CG000185)
• Chromium Single Cell V(D)J Reagent Kits User Guide with Feature Barcoding technology for Cell Surface Protein (CG000186)
• Chromium Next GEM Single Cell 3' Reagent Kits v3.1 User Guide with Feature Barcoding technology for Cell Surface Protein (CG000206)
• Chromium Next GEM Single Cell V(D)J Reagent Kits User Guide v1.1 with Feature Barcoding technology for Cell Surface Protein (CG000208)

To design a custom conjugate oligonucleotide, use a sequence from the Feature Barcode whitelist (CG000193). Please refer to the following article for details: How do I use the Feature Barcode Whitelist?