Next GEM Technology for Single Cell Immune Profiling

Getting Started, Last Modified on June 14, 2022, Permalink

Learn more about the Next GEM Reagents and Chips for the Single Cell Gene Expression Solution

Next GEM is part of 10x Genomics’ commitment to continuous product improvements.

• Next GEM is a technology that is built on a new chip architecture
• Next GEM combines new chips and reagents
• Next GEM reagents are optimized for performance on the Next GEM microfluidic chips

Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1 and Next GEM Chip G Single Cell Kits provide the same features and comparable assay performance as Single Cell V(D)J Reagent Kits v1 and Chip A Single Cell Kits.

• High gene sensitivity, low multiplet rates
• High number of T cells or B cells with productive V-J spanning pairs
• Compatible with Feature Barcoding technology for Cell Surface Protein profiling
• Robust workflow
• Simple chip loading
• Compatible with whole cells
• Industry leading cell capture rates (~65%)
• Broad range of sample throughput (1-8)
• Broad range of cell throughput per sample (100 - 10,000)
• Cell size flexibility, no lower limits

User Guides

• Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1
• Chromium Single Cell V(D)J Reagent Kits v1.1 with Feature Barcoding technology for Cell Surface Protein

Demonstrated Protocols

• Single Cell V(D)J Demonstrated Protocol Compatibility Table
• Sample Preparation

Technical Notes

• Application Note: Performance of Chromium Next GEM Single Cell Immune Profiling Reagent Kits
• Chromium Next GEM Single Cell V(D)J v1.1: Reagent & Workflow Updates

MSDS

• Chromium Next GEM Single Cell V(D)J Library Reagent Kits Safety Data Sheets