10x Genomics Support/Single Cell Immune Profiling/Documentation/Sequencing/

Sequencing Metrics & Base Composition of Single Cell 5' v2 Dual Index Libraries

  • CG000401_TechNote_SeqMetrics_SingleCell5_v2.0_With_BEAM_RevD.pdf

    Technical Note, CG000401

    CG000401_TechNote_SeqMetrics_SingleCell5_v2.0_With_BEAM_RevD.pdf

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The Chromium Next GEM Single Cell 5' v2 (dual index), standard, and High Throughput (HT) workflow produces sequencing-ready Gene Expression, V(D)J, Cell Surface Protein, CRISPR, and/or Barcode Enabled Antigen Mapping (BEAM) libraries from the same single cells. This enables simultaneous profiling of cellular features along with gene expression profiling. This Technical Note presents a comparison of sequencing metrics for various Single Cell 5' v2 Dual Index library types across Illumina platforms. Results may vary depending on the sequencing instrument and/or sample and loading characteristics.

FOR USE WITH:

  • Chromium Next GEM Single Cell 5' Kit v2, 16 rxns PN-1000263
  • Chromium Next GEM Single Cell 5' Kit v2, 4 rxns PN-1000265
  • Library Construction Kit, 16 rxns PN-1000190
  • 5' Feature Barcode Kit, 16 rxns PN-1000256
  • Chromium Single Cell Human TCR Amplification Kit, 16 rxns PN-1000252
  • Chromium Single Cell Human BCR Amplification Kit, 16 rxns PN-1000253
  • Chromium Single Cell Mouse TCR Amplification Kit, 16 rxns PN-1000254
  • Chromium Single Cell Mouse BCR Amplification Kit, 16 rxns PN-1000255
  • Chromium Next GEM Chip K Single Cell Kit, 48 rxns PN-1000286
  • Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287
  • Dual Index Kit TT Set A, 96 rxns PN-1000215
  • Dual Index Kit TN Set A, 96 rxns PN-1000250
Document Type
Technical Note

Last Modified
April 11, 2023