Chromium Connect: Consistent Automated Single Cell 5' Gene Expression and V(D)J Library Generation

Technical Note, Last Modified on December 2, 2020, Permalink


Chromium Connect automates the preparation of sequencing-ready, single cell 5' Gene Expression and V(D)J libraries from the same input cell sample, minimizing technical variation in single cell gene expression data and immune repertoire profiling. Generation of Chromium Single Cell 5’ Gene Expression and V(D)J libraries on the Chromium Connect instrument includes automated Gel Beads-in-emulsion (GEM) generation, barcoding, and library preparation from single cell suspensions, along with additional functionalities for library quantification and pooling. This Technical Note highlights the consistency and reproducibility of single cell gene expression and V(D)J data derived from libraries generated using the Chromium Connect automated workflow.