Cell Labeling with Dextramers for Single Cell RNA Sequencing Protocols

Demonstrated Protocol, Last Modified on October 28, 2020, Permalink


20190828_CG000203 DextramerLabeling_RevAtoRevB.pdf

Multimeric MHC peptide complexes, such as Dextramer reagents, bind to T-cell receptors (TCRs) with high affinity, which can enable detection of TCR antigen specificity. This protocol provides guidance for labeling cells with dCODE™ Dextramer® reagents (Dextramer MHC-Feature Barcode oligonucleotide conjugate), along with guidance for enriching Dextramer reagent+ T cells by Fluorescence Activated Cell Sorting (FACS). These Dextramer reagents labeled cells can be used for generating Chromium Single Cell libraries as described in the User Guide for Chromium Single Cell Immune Profiling Solution with Feature Barcoding technology (CG000186, CG000208 and CG000330).

Antigen-only analysis is not currently supported by 10x Genomics. For antigen analysis, we recommend staining with dextramer reagents in conjunction with a small panel of TotalSeq-C antibodies. This increases the cell calling accuracy of the data. Please contact 10x Genomics support at [email protected] for TotalSeq-C antibody recommendations.