Cell Labeling with dCODE Dextramer® for Single Cell RNA Sequencing Protocols

Demonstrated Protocol, Last Modified on September 9, 2021, Permalink

CG000203_DemonstratedProtocol_CellLabelingwithDextramers_RevC.pdf

20190828_CG000203 DextramerLabeling_RevAtoRevB.pdf

Multimeric MHC peptide complexes, such as dCODE™ Dextramer® reagents, bind to T-cell receptors (TCRs) with high affinity, which can enable detection of TCR antigen specificity. This protocol provides guidance for labeling cells with dCODE™ Dextramer® reagents (dCODE™ Dextramer® MHC-Feature Barcode oligonucleotide conjugate) along with TotalSeq-C antibody-oligonucleotide conjugates. This document also provides guidance for enriching dCODE™ Dextramer®+ T cells by Fluorescence Activated Cell Sorting (FACS). These dCODE™ Dextramer® reagents and TotalSeq-C antibody-oligonucleotide conjugate labeled cells can be used for generating Chromium Single Cell libraries as described in the User Guide for Chromium Single Cell Immune Profiling Solution with Feature Barcode technology (CG000186, CG000208, CG000330, and CG000424).

Antigen-only analysis is not currently supported by 10x Genomics. For antigen analysis, we recommend staining with dCODE™ Dextramer® reagents in conjunction with a small panel of TotalSeq-C antibodies. This increases the cell calling accuracy of the data. Please contact 10x Genomics support at [email protected] for TotalSeq-C antibody recommendations.

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