Single Cell Immune Profiling Datasets

5' Targeted Mouse Demonstration Data (v2 Chemistry)

The links below represent a whole transcriptome analysis and targeted analysis from the same sample.

Cell Ranger 6.0.1

• Mouse Splenocytes: 5’v2 Whole Transcriptome Analysis
• Mouse Splenocytes: 5’v2 Targeted, Custom Immunology Panel

Chromium Connect Demonstration - Human PBMCs (v2 Chemistry)

The links below represent a single cellranger multi run.

Cell Ranger 6.0.1

• Integrated GEX and VDJ analysis of Connect generated library from human PBMCs - Inputs/Library
• Integrated GEX and VDJ analysis of Connect generated library from human PBMCs - single channel

Chromium Connect Demonstration - Human Melanoma (v2 Chemistry)

The links below represent a single cellranger multi run.

Cell Ranger 6.0.1

• Integrated GEX and VDJ analysis of Connect generated library from human melanoma - Inputs/Library
• Integrated GEX and VDJ analysis of Connect generated library from human melanoma - single channel

Chromium Connect Demonstration - Mouse PBMCs (v2 Chemistry)

The links below represent a single cellranger multi run.

Cell Ranger 6.0.1

• Integrated GEX and VDJ analysis of Connect generated library from mouse PBMCs - Inputs/Library
• Integrated GEX and VDJ analysis of Connect generated library from mouse PBMCs - single channel

Chromium Connect Demonstration - Mouse Splenocytes (v2 Chemistry)

The links below represent a single cellranger multi run.

Cell Ranger 6.0.1

• Integrated GEX and VDJ analysis of Connect generated library from mouse splenocytes - Inputs/Library
• Integrated GEX and VDJ analysis of Connect generated library from mouse splenocytes - single channel

Human B cells from a Healthy Donor, 1k cells - multi (v2 Chemistry)

This is the same data previously analyzed in Cell Ranger 5.0.0.

The links below represent a single cellranger multi run.

Cell Ranger 6.0.0

• Human B cells from a Healthy Donor, 1k cells - multi (v2) - Inputs/Library
• Human B cells from a Healthy Donor, 1k cells - multi (v2) - Human B cells

V(D)J + 5' Gene Expression Demonstration (v2 Chemistry)

Cell Ranger 5.0.0

• Human B cells from a healthy donor post-flu vaccination (v2)
• Human B cells from a healthy donor pre-flu vaccination (v2)
• Human B cells from a healthy donor pre- and post- flu vaccination (v2)
• Human T cells from a Healthy Donor, 1k cells - multi (v2)
• Human B cells from a Healthy Donor, 1k cells - multi (v2)
• T cells from BALB/c Mice 1k cells - multi (v2)
• B cells from BALB/c Mice 1k cells - multi (v2)
• Human PBMC from a Healthy Donor, 10k cells - multi (v2)
• Human PBMC from a Healthy Donor, 10k cells (v2)

Cell Ranger 4.0.0

• Melanoma Tumor Derived Cells (v1.1)
• Melanoma Tumor Derived Cells (v2)
• Splenocytes from C57BL/6 mice, 1k cells (v1.1)
• Splenocytes from C57BL/6 mice, 1k cells (v2)
• Splenocytes from C57BL/6 mice, 10k cells (v2)
• Human PBMC from a Healthy Donor, 1k cells (v1.1)
• Human PBMC from a Healthy Donor, 1k cells (v2)
• Human PBMC from a Healthy Donor, 10k cells (v2)

5' Targeted Neuroscience Demonstration Data (v1 Chemistry)

Cell Ranger 4.0.0

• Human Glioblastoma Multiforme: 5’v1 Whole Transcriptome Analysis
• Human Glioblastoma Multiforme: 5’v1 Targeted, Neuroscience Panel

5' Targeted Demonstration (v2 Chemistry)

Cell Ranger 4.0.0

• Melanoma, Dissociated Tumor Cells: Targeted, Human Pan-Cancer Panel
• Melanoma, Dissociated Tumor Cells: Targeted, Human Immunology Panel
• Melanoma, Dissociated Tumor Cells: Targeted, Human Gene Signature Panel

5' Targeted Demonstration (v1.1 Chemistry)

Cell Ranger 4.0.0

• PBMCs from a Healthy Donor: Whole Transcriptome Analysis
• PBMCs from a Healthy Donor: Paired T Cell Receptor
• PBMCs from a Healthy Donor: Targeted, Immunology Panel
• PBMCs from a Healthy Donor: Targeted-Compare, Immunology Panel

V(D)J + 5' Gene Expression Next GEM Demonstration (v1.1 Chemistry)

Cell Ranger 3.1.0

• Human PBMC - 5' Cell Surface Protein-Only (v1)
• Human PBMC - 5' Cell Surface Protein-Only (Next GEM v1.1)
• PBMCs of a Healthy Donor (v1)
• PBMCs of a Healthy Donor (Next GEM v1.1)
• PBMCs from C57BL/6 mice (v1)
• PBMCs from C57BL/6 mice (Next GEM v1.1)
• PBMCs of a Healthy Donor (v1, 150x150)
• PBMCs of a Healthy Donor (Next GEM v1.1, 150x150)
• PBMCs from C57BL/6 mice (v1, 150x150)
• PBMCs from C57BL/6 mice (Next GEM v1.1, 150x150)

Application Note - A New Way of Exploring Immunity

Datasets for the Application Note “A New Way of Exploring Immunity - Linking Highly Multiplexed Antigen Recognition to Immune Repertoire and Phenotype”. Assess the binding specificities of over 150,000 CD8+ T cells from 4 human donors across a highly multiplexed panel of 44 distinct, specific peptide–MHC (pMHC) multimers.

Cell Ranger 3.0.2

• CD8+ T cells of Healthy Donor 1
• CD8+ T cells of Healthy Donor 2
• CD8+ T cells of Healthy Donor 3
• CD8+ T cells of Healthy Donor 4

Application Note - Redefining Cellular Phenotyping

Datasets for the Application Note “Redefining Cellular Phenotyping: Comprehensive Characterization and Resolution of the Antigen-Specific T Cell Response”. Perform multiomic characterization of immune cells by linking multiple attributes such as surface protein, gene expression, and antigen interactions to the expressed TCR clonotype from CMV seropositive and seronegative human donors.

Cell Ranger 5.0.0

• Sorted PBMCs from a CMV seropositive donor
• Unsorted PBMCs from a CMV seropositive donor
• Aggregated PBMCs from a CMV seropositive donor
• Sorted PBMCs from a CMV seronegative donor
• Unsorted PBMCs from a CMV seronegative donor
• Aggregated PBMCs from a CMV seronegative donor

Cell Ranger 3.0.2

• Sorted PBMCs from a CMV seropositive donor - 5' gene expression and cell surface protein
• Sorted PBMCs from a CMV seropositive donor - TCR enrichment from amplified cDNA
• Unsorted PBMCs from a CMV seropositive donor - 5' gene expression and cell surface protein
• Unsorted PBMCs from a CMV seropositive donor - TCR enrichment from amplified cDNA
• Aggregated PBMCs from a CMV seropositive donor - 5' gene expression and cell surface protein
• Sorted PBMCs from a CMV seronegative donor - 5' gene expression and cell surface protein
• Sorted PBMCs from a CMV seronegative donor - TCR enrichment from amplified cDNA
• Unsorted PBMCs from a CMV seronegative donor - 5' gene expression and cell surface protein
• Unsorted PBMCs from a CMV seronegative donor - TCR enrichment from amplified cDNA
• Aggregated PBMCs from a CMV seronegative donor - 5' gene expression and cell surface protein

V(D)J + 5' Gene Expression + Cell Surface Protein - Human

Cell Ranger 3.0.0

• PBMCs of a healthy donor - 5' gene expression and cell surface protein
• PBMCs of a healthy donor - TCR enrichment from amplified cDNA
• PBMCs of a healthy donor - Ig enrichment from amplified cDNA

V(D)J + 5' Gene Expression - Mouse

Cell Ranger 3.0.0

• PBMCs from C57BL/6 mice - 5’ gene expression
• PBMCs from C57BL/6 mice - TCR enrichment from amplified cDNA
• PBMCs from C57BL/6 mice - Ig enrichment from amplified cDNA
• PBMCs from BALB/c mice - 5’ gene expression
• PBMCs from BALB/c mice - TCR enrichment from amplified cDNA
• PBMCs from BALB/c mice - Ig enrichment from amplified cDNA

V(D)J + 5' Gene Expression - Human

Cell Ranger 5.0.0

• NSCLC tumor

Cell Ranger 2.2.0

• NSCLC tumor - 5’ gene expression
• NSCLC tumor - TCR enrichment from amplified cDNA
• NSCLC tumor - Ig enrichment from amplified cDNA