Interpreting Cell Ranger Multi Web Summary Files for Single Cell 3’ Gene Expression with Feature Barcode technology for Cell Multiplexing

Technical Note, Last Modified on November 15, 2021, Permalink

CG000475_TechNote_ChromiumNextGEM_SC3'_CMOWebSummary_Rev A.pdf

The web summary file (web_summary.html) output by the cellranger multi pipeline is the initial point of reference for determining sample performance in the Chromium Single Cell 3’ Gene Expression with Feature Barcode technology for Cell Multiplexing assay. This Technical Note presents an overview of web summary file interpretation, including the expected metrics and characteristic plots for libraries generated using this assay.