Chromium Single Cell 3’ v2 Libraries – Sequencing Performance on Illumina® NextSeq® 500 Flow Cells

Technical Note, v2 Chemistry, Last Modified on November 19, 2018, Permalink

CG000085_Technical Note_Sequencing Metrics for NextSeq 500.pdf

Technical Note - Chromium™ Single Cell 3’ v2 Libraries – Sequencing Performance on Illumina® NextSeq® 500 Flow Cells

This Technical Note presents a comparison of sequencing metrics for Single Cell 3’ v2 libraries run on NextSeq 500 Illumina flow cells. While sequencing metrics differ between sequencing platforms (e.g. MiSeq versus HiSeq 4000), they are generally consistent across runs on the same platform. We have found, however, that Single Cell 3’ v2 libraries sequenced on NextSeq 500/550 can show significant variability in sequencing metrics (in particular, Q30 score for Read 2) across different flow cell lots.

FOR USE WITH