Technical Note, v2 Chemistry, Last Modified on November 19, 2018, Permalink
Technical Note - Chromium™ Single Cell 3’ v2 Libraries – Sequencing Performance on Illumina® NextSeq® 500 Flow Cells
This Technical Note presents a comparison of sequencing metrics for Single Cell 3’ v2 libraries run on NextSeq 500 Illumina flow cells. While sequencing metrics differ between sequencing platforms (e.g. MiSeq versus HiSeq 4000), they are generally consistent across runs on the same platform. We have found, however, that Single Cell 3’ v2 libraries sequenced on NextSeq 500/550 can show significant variability in sequencing metrics (in particular, Q30 score for Read 2) across different flow cell lots.
FOR USE WITH