Resolving Cell Types as a Function of Read Depth and Cell Number

Technical Note, Last Modified on February 27, 2018, Permalink

CG000148_10x_Technical_Note_Resolving_Cell_Types_as_Function_of Read_Depth_Cell_Number_RevA.pdf

Technical Note - Resolving Cell Types as a Function of Read Depth and Cell Number

Chromium™ Single Cell Solutions enable gene expression profiling of 100–10,000 cells per reaction. When sequencing costs or capacity are limiting, there is often a trade-off between sequencing a larger cell number (breadth) and sequencing a smaller cell number with more reads (depth). Here, we examine how sequencing depth and cell number influence the detection of major cell types in peripheral blood mononuclear cells (PBMCs).

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