Quality Control of Cell Surface Protein Labeling using Flow Cytometry

Technical Note, Feature Barcoding technology, Last Modified on August 9, 2021, Permalink


Cell surface proteins can be labeled with a specific protein binding molecule, such as an antibody conjugated to a Feature Barcode oligonucleotide, to generate Chromium Single Cell 3' or 5' Cell Surface Protein libraries. This Technical Note outlines methods to perform quality control of cell surface protein labeling with antibody-oligonucleotide conjugates using flow cytometry, along with guidelines for determining optimal labeling concentration.