Cryopreservation is a common method for preserving samples for long-term storage and transport. However, some cell types may be sensitive to freeze-thaw cycles or may need to be stored for only a few hours, highlighting a need for alternative methods of sample preservation. Protocols for shortterm storage of cells without cryopreservation are limited, and the impact of these on single cell sequencing data has not been thoroughly evaluated. This Technical Note evaluates HypoThermosol FRS and RPMI + 10% FBS as short-term cell storage solutions in 10x Genomics Single Cell 3' Gene Expression Assays.