Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing

Demonstrated Protocol, Last Modified on May 11, 2022, Permalink

CG00054_SamplePrepDemonstratedProtocol - CultCells_RevB.pdf

This Demonstrated Protocol outlines (i) how prepare single cell suspensions from cultured cell lines in preparation for use in 10x Genomics Single Cell Protocols.

This demonstrated protocol is optimized for counting cells in the range of 700-1200 cells/µl. If using the Single Cell 3' LT v3.1 (low throughput) application, ensure cells are counted as indicated in this protocol and then diluted to the LT specific optimal loading concentration of 100-600 cells/µl using the Cell Dilution Overview in the LT User Guide.