Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing

Demonstrating Single Cell Behavior Using Human-Mouse Cell Line Mixtures

Demonstrated Protocol, Last Modified on September 17, 2019, Permalink

CG00014_Demonstrated_Protocol_FreshFrozenHumanMouseCellLineMix_RevF .pdf


The ability of the 10x GenomicsĀ® Single Cell Solutions to partition single cells in a heterogeneous population can be verified by profiling a mixture of cells from two different species. Ideally, all sequence reads from a single Gel Bead-in-EMulsion (GEM) will be unambiguously mapped to the transcriptome of only one of the two species.