Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing

Demonstrating Single Cell Behavior Using Human-Mouse Cell Line Mixtures

Demonstrated Protocol, Last Modified on June 29, 2017, Permalink

CG00014_SamplePrepDemonstratedProtocol - H-MCellMix_RevE.pdf

The ability of the 10x GenomicsĀ® Single Cell Solutions to partition single cells in a heterogeneous population can be verified by profiling a mixture of cells from two different species. Ideally, all sequence reads from a single Gel Bead-in-EMulsion (GEM) will be unambiguously mapped to the transcriptome of only one of the two species.