Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols
with Feature Barcode technology
Demonstrated Protocol, Last Modified on March 3, 2021, Permalink
The 10x Genomics 3’ CellPlex Kit provides a species-agnostic
sample multiplexing solution through the use of a set of 12
Feature Barcode oligonucleotides each conjugated to a lipid.
These Cell Multiplexing Oligos can be used to label individual
cells or nuclei samples, and the labeled cells can be pooled
together prior to loading onto a 10x Genomics chip. The Feature
Barcode molecules can be directly captured by oligonucleotides
present on the Gel Beads inside a GEM during GEM-RT,
subsequently amplified, and used to generate Cell Multiplexing
This protocol provides guidance for:
- Labeling cells/nuclei with CellPlex reagents (see page 2-3, Cell Multiplexing Oligo Labeling) for use with Single Cell 3'v3.1 (Dual Index) protocols with Feature Barcode technology
for Cell Multiplexing (CG000388) and Single Cell 3' v3.1 (Dual
Index) protocols with Feature Barcode technology for CRISPR
Screening & Cell Multiplexing (CG000389).
- Labeling cells with antibody-oligonucleotide conjugates and
CellPlex reagents (see page 4-5, Cell Surface Protein & Cell
Multiplexing Oligo Labeling) for use with Single Cell 3' v3.1
(Dual Index) protocols with Feature Barcode technology for
Cell Surface Protein & Cell Multiplexing (CG000390).