Chromium Nuclei Isolation Kit Tested Tissues

10x Genomics Sample Preparation Products, Chromium Nuclei Isolation Kit, Last Modified on June 10, 2022, Permalink

The sample types listed below are frozen tissues that were tested with the Chromium Nuclei Isolation Kit. All samples were tested with no modification to Chromium Nuclei Isolation workflow.

Compatible Tissues

All tissues listed in this table consistently achieved high quality data. Nuclei yield and final data quality are dependent on tissue handling and storage, starting tissue quality, and disease state.

Organism Tissue Disease State
Mouse Brain Healthy
Mouse Heart Healthy
Mouse Lung Healthy
Mouse Kidney Healthy
Mouse Liver Healthy
Mouse Small Intestine Healthy
Mouse Eye Healthy
Mouse Skeletal Muscle (Tibialis Anterior) Healthy
Mouse Testis Healthy
Mouse Ovary Healthy
Mouse Spinal Cord Healthy
Mouse Colon Healthy
Mouse Adipose Tissue Healthy
Mouse Bladder Healthy
Mouse Stomach Healthy
Human Brain Healthy
Human Jejunum Healthy
Human Duodenum Healthy
Human Ileum Healthy
Human Testis Healthy
Human Kidney Tumor *
Human Breast Tumor *
Human Large Intestine Tumor *
Human Lung Tumor *
Human Melanoma Tumor *
Human Ovary Tumor *
Human Pancreas Tumor *
Human Prostate Tumor *

* Tumors are heterogeneous and variability in data quality is expected. Biological variability between patients or disease states impact data quality.

Tissues with variable results

Tissues listed in this table generated variable outputs in nuclei yield or data quality. Most of these samples are known to be challenging.

Organism Tissue Challenging Phenotype
Mouse Skin Low nuclei yield
Mouse Tongue Low nuclei yield
Mouse Pancreas High RNase content
Mouse/Human Spleen High RNase content

Incompatible Tissues

These sample types are not supported or compatible with the Chromium Nuclei Isolation Kit. We do not recommend these types of samples to be run with this kit.

Incompatible Samples
Cell suspensions (i.e. cultured cells, PBMCs)
Calcified tissue (i.e. bone)
Plant tissue
Insect tissue
Fixed Tissue (e.g. FFPE, PFA)

The list of tissues tested reflect our access to these sample types and the specific tissues tested were chosen to reflect the wide range of possible input tissues expected to be used with this kit.

If your tissue of interest is not present in the list of tissues tested, please refer to the following article for guidance: What if my tissue is not on the list of tissues tested with the Chromium Nuclei Isolation Kit?