Technical Note, v3 Chemistry with Feature Barcoding technology, Last Modified on November 19, 2018, Permalink
The Single Cell Gene Expression Solution coupled with Feature Barcoding technology enables profiling of gene expression in conjunction with additional cellular features from the same single cell. Specifically, the Single Cell Gene Expression and CRISPR Screening Solution provides a high-throughput and scalable approach to obtain gene expression profiles along with CRISPR-mediated perturbation phenotypes in transduced cells via direct capture of poly-adenylated mRNAs and guide RNAs (sgRNAs). This document provides specifications for engineering Feature Barcoding technology compatible sgRNAs for use with:
