Single Cell Gene Expression Demonstrated Protocol Compatibility Table

Demonstrated Protocol, Last Modified on June 27, 2019, Permalink

Protocol SC3'v2 SC3'v3/v3.1 SC3'v3/v3.1 with CRISPR Screening SC3'v3/v3.1 with Cell Surface Protein
Single Cell Protocols - Cell Preparation Guide
Methanol Fixation of Cells for Single Cell RNA Sequencing ✓*
Isolation of Nuclei for Single Cell RNA Sequencing ✓* -
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling ✓* ✓* -
Tumor Dissociation for Single Cell RNA Sequencing ✓* -
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing ✓* ✓* ✓*
Moss Protoplast Suspension for Single Cell RNA Sequencing ✓*
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing ✓*
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing ✓* ✓*
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols ** -

✓ Compatible (tested in-house)
✓* Untested but low risk
** Compatible but unsupported
- Untested, use at your own risk
✘ Incompatible